Hello! I have this project Due Monday, August 2nd. I will attach all four parts to this submission. Please let me know if this is possible. Looking for the best to have this completed by Monday night. 


Biology 1114 Research Project Worksheet
Answer all questions, written in bold (10 points). This worksheet is designed to help you organize your thoughts about this experiment and to facilitate writing your report.

-How are your beetles classified (to which class, order and family do they belong)?

-How many adult beetles will be placed in each jar?

-Write here what the amounts of food in each jar will be:

-How much vermiculite will be in each jar?

-What are we trying to find out?

Predictions- Make predictions on how your beetle populations will react to changing food and space conditions.

Experimental Methods- What is the difference between a control and experimental group?

Which jar is the control jar and which are the experimental ones?

Independent and dependent variables:

The independent variable is the factor being manipulated or changed by the researcher.

The dependent variable is the product of changing the independent variable, these are your results.

Let’s try an example. Imagine you feed two groups of white flamingoes with different diets. One group feeds on fish, the other group feeds on shrimp. You hypothesize the group feeding on shrimp will turn a pink color.

What are the independent and dependent variables in the shrimp and flamingo example?

Now name the independent and dependent variables in the beetle exercise.

Control Variables. When doing an experiment, we want to make sure we are testing only the variables we manipulate. So, we have to keep other living conditions uniform. These are called control variables and they should not change throughout the experiment. For example, in an experiment of coral growth, we would want to keep the water temperature and amount of light the same throughout the course of the experiment.

Name some control variables (variables that should not change) in our jars:

Determine the null and alternative hypotheses. The null hypothesis is the one we test, the alternative hypothesis contradicts the null hypothesis, it is probably the one we favor. We accept or reject the null hypothesis based on the outcomes of the experiment. In actual practice, it is difficult to prove the null hypothesis is true, so if we reject it, then our alternative hypothesis gains credibility.

Let’s try an example. You are testing the number of times that young lobsters leave their hiding places and search for food each hour during the evening. You feel that lobsters in dark tanks will make more searches per hour than lobsters in well lighted tanks.

Your null hypothesis will be, “there are no differences in the number of searches by young lobsters in tanks that are either dark or well lighted.” Your alternative hypothesis will be, “there are differences in the number of searches by young lobsters in tanks that either dark or well lighted.”

Write your null and alternative hypotheses for the beetle experiment. Since we have two experimental jars, we have t

Biology 1114 Research Project Report Instructions

You will turn in both a worksheet and a research paper.
Point total for research paper: 90 points
Point total for worksheet: 10 points
Your paper will be written in the format of a scientific journal article and include the following parts:

Title (2 points): The title of a scientific paper is specific and tells the reader everything he/she needs to know in order to decide if this paper is relevant to his or her own work.

Abstract (8 points): This is a summary of the entire project, all in about one paragraph. It includes the results, so you can’t write it until you are done with the project.

Introduction (15 points): Talk about the species of beetle we will be using. Discuss its ecological niche presently as well as what it likely was before humans began storing grain. How does Tribolium confusum interact with other species in its environment including humans. You will need to include the food habits, life history, and rates of reproduction for this beetle. You will also need to determine the acceptable ranges of temperature, humidity, and how this beetle reacts to light. Cite your references by naming the authors with their publication years such as (Jones, 2004). This section should be relatively short and to the point. At the end of the introduction, tell what the hypotheses are (both null and alternative). Give a prediction of the results.

Methods (15 points): Describe the experimental methods, tell what was measured and how. Tell when it was measured. List all your equipment and the steps you used to setup and complete the experiment. The idea of a methods section is to be detailed enough that someone else could perform the experiment from your description. DO NOT INCLUDE ANY RESULTS IN THIS SECTION.

Results (15 points): Here is where you will present your experimental data in the form of tables and/or graphs. In this section you provide your results, but you do not yet draw conclusions from them. That effort is left for your last section. You must also test your results using a statistical test and make sure you report these results and the methods you used to obtain them.

Conclusions (15 points): This is where you discuss your results and draw conclusions (i.e. what might have caused any differences observed in the containers. Also, in this section you accept or reject your hypotheses. This is where you discuss the possibility of experimental errors.

Writing Style (5 points): The writing style in a scientific paper is described as “detached third person.” You are not offering opinions or comment. Avoid words such as I, you, we, because you are merely collecting and reporting facts. Spelling, grammar, and the organization of your ideas will be parts of your grade on this paper, so please pay attention to your writing. Seek help (from me, other students, the writing center on campus) if you are unsure about how to write in a scientific style. Avoid using bullets! If Eng

Biology 1114 Research Project Experimental Set-up and Overview
General: Flour beetles (Tribolium confusum), also known as the confused beetle, will be raised in plastic containers for approximately seven to eight weeks. The amounts of food and space will be varied in each of these containers which may impact the densities of the beetle. Space is provided for the beetles by the addition of vermiculite to two of the three containers. The addition of this material will allow the beetles to move away from one another. The populations in each container will be evaluated at the end of this study by counting numbers of live adults, pupae, and larvae. Two different hypotheses will be tested, each of which will be based on amount of food and space in the containers. At the conclusion of this experiments, you will present the results in written report.
Experimental Procedures
Three rearing containers will be set up in the following manner:

Container A [Control]: 25 adult beetles, 25 grams of food, enough vermiculite to fill the container to the top (control group)

Container B [Experiment 1]: 25 adult beetles, 25 grams of food, no vermiculite (high density of beetles)

Container C [Experiment 2]: 25 adult beetles, 10 grams of food, enough vermiculite to fill the container half full. (low density of beetles)

1. Groups of students will be assigned a container to prepare. The outside of each container will need to be labeled with a letter (A, B, or C) as well as the date that the experiment was started. Plastic weigh boats will be used to weigh out the food that will be added to each container. Make sure to zero scales after placing weigh boats on them. Put the appropriate amount of food in each container. After the food has been added, you will need to sort through the material in the beetle cultures in order to locate live adults for your experiments. The next step is for you to deposit 25 healthy adult beetles in each of the plastic rearing containers. The experimental design requires the addition of different amounts of vermiculite to container A and C. Add the vermiculite to the appropriate level in these two containers.
2. The containers will need to be sealed tightly so that the beetles do not escape. Cut a piece of cheese cloth large enough so that when it is folded in half horizontally and vertically will still amply cover the top of the container. Place a paper towel over the cheese cloth. Use several elastic bands to secure the cheese cloth/paper towel covering. Once this is done, the containers will need to be placed in an incubator for the duration of the experiment.

Biology 1114 Research Project: Sorting and Data Recording
After seven to eight weeks, the beetles in each of the experimental containers need to be counted. You will encounter three life stages, adults, larvae, and pupae and each of these needs to be counted separately.
These life stages are figured below

The two containers with vermiculite (A, C) will be the most time consuming to sort through. In these containers, most of the live material will likely be found near the bottom. All of these containers will contain many live beetles. The easiest life stage to recognize and find is the adult. Adult beetles are usually dark-colored, active, and they look like the figure above. The larvae are pupae pale are not as easy to locate and identify. They are pale and resemble small meal worms. Early instar larvae are tiny and easily overlooked. The pupae are also pale and are about the same size as the adult beetles but the pupae are mostly inactive. The only movable part of the pupa is its abdomen. Because larvae and pupae are not conspicuous, it is of the utmost importance to sort through only
amounts of material from the contains at a time. The first step is to visually examine the material from the containers. In order to do this, you will need a chemical scoop and a sorting tray. Use the scoop to deposit a small pile of material on the left side of your sorting tray. Next, use a small paintbrush to gradually move some of this material to the right in order to locate any live material. Always look for movement in the substrate you are examining. Once the material has been visually examined, it should then be re-examined under a dissecting scope. By doing this, you will likely encounter tiny larvae that were missed during your initial sort. You will need to keep track of all the adults, larvae, and pupae you find within a container. You may encounter dead adults during the sorting procedure. Simply add the number of dead adults to the number of live adults that you find.


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